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鏈夋晥鏈燂細6鍊�(g猫)鏈�

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瀵�(sh铆)椹�(y脿n)鍘熺悊

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妯ｆ湰铏曠悊鍙婅姹�

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3.  绲勭箶鍕绘伎锛�鐢ㄩ爯(y霉)鍐风殑PBS (0.01M, pH=7.4)娌栨礂绲勭箶锛屽幓闄ゆ畼鐣欒娑诧紙鍕绘伎涓瑙ｇ殑绱呯窗(x矛)鑳炴渻(hu矛)褰遍熆娓噺绲�(ji茅)鏋滐級锛岀ū閲嶅悗灏囩祫绻斿壀纰�銆傚皣鍓鐨勭祫绻旇垏灏�(du矛)鎳�(y墨ng)楂旂⿳鐨�PBS锛堜竴鑸寜1:9鐨勯噸閲忛珨绌嶆瘮锛屾瘮濡�1g鐨勭祫绻旀ǎ鍝佸皪(du矛)鎳�(y墨ng)9mL鐨�PBS锛屽叿楂旈珨绌嶅彲鏍规摎(j霉)瀵�(sh铆)椹�(y脿n)闇€瑕侀仼鐣�(d膩ng)瑾�(di脿o)鏁�锛屽苟鍋氬ソ瑷橀寗銆傛帹钖﹀湪PBS涓姞鍏ヨ泲鐧介叾鎶戝埗鍔戯級鍔犲叆鐜荤拑鍕绘伎鍣ㄤ腑锛屼簬鍐颁笂鍏呭垎鐮旂（銆傜偤浜嗛€�(j矛n)涓€姝ヨ瑙ｇ祫绻旂窗(x矛)鑳�锛屽彲浠ュ皪(du矛)鍕绘伎娑查€�(j矛n)琛岃秴鑱茬牬纰�锛屾垨鍙嶅京(f霉)鍑嶈瀺銆傛渶鍚庡皣鍕绘伎娑蹭簬5000脳g闆㈠績5~10鍒嗛悩锛屽彇涓婃竻妾㈡脯銆�

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闇€瑕佽€屾湭鎻愪緵鐨勮│鍔戝拰鍣ㄦ潗

1.  閰舵(bi膩o)鍎€锛�450nm锛�

2.  楂樼簿搴﹀姞妯ｅ櫒鍙婃闋細0.5-10uL銆�2-20uL銆�20-200uL銆�200-1000uL

3.  37鈩�鎭掓韩绠�

4.  钂搁ぞ姘存垨鍘婚洟瀛愭按

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3.  娑堥櫎鏉垮簳娈樼暀鐨勬恫楂斿拰鎵嬫寚鍗帮紝鍚﹀墖褰遍熆OD鍊�銆�

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鎿嶄綔姝ラ

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7.  姣忓瓟鍔犲叆绲傛娑�50渭L锛�15min鍏�(n猫i)锛屽湪450nm娉㈤暦铏曟脯瀹氬悇瀛旂殑OD鍊�銆�

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瑭﹀姂鐩掓€ц兘

1.  妾㈡脯鑼冨湇锛�6.25 pg/mL 鈥� 200 pg/mL銆�

2.  闈堟晱搴︼細鏈€浣庢娓績搴﹀皬浜�1 pg/mL銆�

3.  鐗圭暟鎬э細涓嶈垏鍏跺畠鍙憾鎬х祼(ji茅)妲�(g貌u)椤炰技鐗╀氦鍙夊弽鎳�(y墨ng)銆�

4.  閲嶅京(f霉)鎬э細鏉垮収(n猫i)璁婄暟绯绘暩(sh霉)灏忎簬10% 锛�鏉块枔璁婄暟绯绘暩(sh霉)灏忎簬15% 銆�

瑾槑

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1.  鏈€绲傜殑瀵�(sh铆)椹�(y脿n)绲�(ji茅)鏋滆垏瑭﹀姂鐨勬湁鏁堟€�銆佸(sh铆)椹�(y脿n)鑰呯殑鐩搁棞(gu膩n)鎿嶄綔浠ュ強鐣�(d膩ng)鏅�(sh铆)鐨勫(sh铆)椹�(y脿n)鐠�(hu谩n)澧冨瘑鍒囩浉闂�(gu膩n)锛岃珛(q菒ng)鍕�(w霉)蹇呮簴(zh菙n)鍌欏厖瓒崇殑妯�(bi膩o)鏈倷浠�銆�

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