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1
锛庤壊璀滃剙 (甯堕€插彛 鑷嫊鍗侀€氶枼)
2锛庢娓櫒锛欶ID
3锛庤壊璀滄煴锛�
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B.
鍒嗘瀽鏌� 姣涚窗绠℃煴 30m脳0.32mm脳2.65渭m锛屼氦鑱�(li谩n)鐢插熀纭呴叜鐭宠嫳姣涚窗绠℃煴
4锛庤壊璀滃伐浣滅珯
5锛庨厤濂楁埃婧�
6.锛庢婧栨ǎ鍝侊細
A. 娣峰悎閱囬啔鏍℃妯欐ǎ
B. 閱囬啔鍏�(n猫i)妯�
C. 娣峰悎鑺崇兇鏍℃妯欐ǎ
D锛庤姵鐑村収(n猫i)妯�
7.鍒嗘瀽娴佺▼:
7.1 鍚哀鍖栧悎鐗╁垎鏋愶細灏囧収(n猫i)妯欎箼浜岄唶浜岀敳鍩洪啔锛圖ME锛夊姞鍏ユǎ鍝佷腑锛屽叆TCEP闋�(y霉)鍒囨煴锛屾斁绌鸿紩鐑�锛岀劧鍚庡湪鐢插熀鐠�(hu谩n)鎴婄兎鍜岀敳鍩哄彅涓佸熀閱氫箣闁撳垏鎻�锛岃畵鍚哀鍖栧悎鐗╅€插叆WCOT鏌�锛屽緟鍙旀垔鍩虹敳鍩洪啔娴佸嚭鍚�锛屽啀鍙嶅惞閲嶇兇绲勫垎銆�
7.2. 鑺崇兇鍒嗘瀽锛氬皣鍏�(n猫i)妯�2-宸遍叜鍔犲叆妯ｅ搧涓�锛屽叆TCEP闋�(y霉)鍒囨煴锛屾斁绌鸿嫰銆佺敳鑻拰灏忎簬C12鐨勯潪鑺崇兇锛岀劧鍚庡湪涔欒嫰娴佸嚭涔嬪墠鍒囨彌锛岃畵鑺崇兇閫插叆WCOT鏌憋紝寰呴劙浜岀敳鑻祦鍑哄悗锛屽啀鍙嶅惞鍓╀綑绲勫垎锛圕9鍜孋9浠ヤ笂鑺崇兇锛夐€插叆妾㈡脯鍣ㄦ娓�銆�
